Molecular technologies for detection and typing of bluetongue virus
نویسندگان
چکیده
منابع مشابه
Development and Evaluation of Real Time RT-PCR Assays for Detection and Typing of Bluetongue Virus
Bluetongue virus is the type species of the genus Orbivirus, family Reoviridae. Bluetongue viruses (BTV) are transmitted between their vertebrate hosts primarily by biting midges (Culicoides spp.) in which they also replicate. Consequently BTV distribution is dependent on the activity, geographic distribution, and seasonal abundance of Culicoides spp. The virus can also be transmitted verticall...
متن کاملAppling real time RT-PCR for bluetongue virus detection in Iran
During 2009-10, real time RT-PCR and conventional RT-PCR techniques Used for detecting BTVs RNA in 310 blood samples. For real time and gel based RT-PCR segment-1 and segment-10 selected as conserve genes to search any BTV strains. Using these methods, 58 (%18.7) and 14 (%4.5) positive samples were detected among the clinically suspected sheep. Sensitivity of both molecular techniques evalua...
متن کاملSerological and Molecular Detection of Akabane Virus in Iran
The present study was conducted on sixty healthy female domestic ruminants that suffered from still births or abortion, to find sero-positive cases and also to determine the probable role of uterine tissue in diagnosis of akabane viral infection and viral persistency. Because of this, a serological test (ELISA) and molecular (RT-PCR) diagnostic methods were used to find the seropositive cases a...
متن کاملBluetongue virus replication, molecular and structural biology.
The icosahedral bluetongue virus (BTV) particle (~80 nm diameter) is composed of three distinct protein layers. These include the subcore shell (VP3), core-surface layer (VP7) and outer capsid layer (VP2 and VP5). The core also contains ten dsRNA genome segments and three minor proteins (VP1[Pol], VP4[CaP]and VP6[Hel]), which form transcriptase complexes. The atomic structure of the BTV core ha...
متن کاملEnterovirus molecular detection and typing
Cell culture and neutralization have been considered the gold standard for enterovirus detection and identification for more than 50 years, but molecular amplification technologies are rapidly replacing the traditional methods in clinical and public health laboratories. Assays based on reverse transcription–polymerase chain reaction or nucleic acid sequence-based amplification may be used to de...
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ژورنال
عنوان ژورنال: Medycyna Weterynaryjna
سال: 2017
ISSN: 0025-8628
DOI: 10.21521/mw.5698